Ph.D. University of California San Francisco, Department of Biochemistry and Biophysics, Program in Biological Sciences 2000-2008
B.A. Grinnell College, Majors in Biology and Philosophy, 1996-2000
Recently, we found that one such complex, the spliceosome, can be regulated to rapidly alter the splicing efficiency of specific pre-mRNA transcripts, thereby shifting the cellular population of mature, translatable messages in the yeast Saccharomyces cerevisiae. This discovery was made using a custom microarray platform which allowed us to assay the relative splicing efficiency of every intron-containing transcript in the yeast genome. Through a collaborative effort, we have built a database of thousands of microarray-based splicing profiles representing dozens of unique environmental conditions and hundreds of different mutations in mRNA processing factors.
Across a wide range of different environmental contexts we see evidence of rapid, transcript-specific changes in splicing efficiency. The splicing of transcripts can be both up-regulated and down-regulated in response to environmental cues. In some cases large numbers of transcripts are affected, in others only defined subsets. In total, our data reveal that splicing is a remarkably dynamic and flexible mechanism for post-transcriptional gene regulation in yeast.
BIOL-111: Fundamentals of Biology, "Genes, Drugs, and Toxins"
BIOL-215: Biochemistry of the Cell
At Grinnell College:
BIO-150: Introduction to Biological Inquiry, "Genes, Drugs, and Toxins"
BIO-251: Molecules, Cells, & Organisms, with Lab
BIO-345: Advanced Genetics, with Lab
BIO-370: Advanced Cell Biology, with Lab
BIO-395: Advanced Special Topic, "Systems Biology"
BCM-262L: Introduction to Biological Chemistry - Lab
|Diverse environmental stresses elicit distinct responses at the level of pre-mRNA processing in yeast.||RNA||Bergkessel, M.*, G.B. Whitworth* and C. Guthrie. (2011) RNA 17: 1461-78.|
|An Introduction to Microarray Data Analysis and Visualization||Abstract||Gregg B. Whitworth. In John Abelson and Melvin Simon editors: METHODS IN ENZYMOLOGY, Vol. 470, Guide to Yeast Genetics: Functional Genomics, Proteomics, and Other Systems Analysis, Jonathan Weissman, Christine Guthrie and Gerald R. Fink, Burlington: Academic Press, 2010, pp.19-50.|
|A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing.||PubMed Central||Wilmes, G.M., M. Bergkessel, S. Bandyopadhyay, M. Shales, H. Braberg, G. Cagney, S.R. Collins, G.B. Whitworth, T.L. Kress, J.S. Weissman, T. Ideker, C. Guthrie, and N.J. Krogan. (2008) Molecular Cell 32: 735-46.|
|Genome-wide search for yeast RNase P substrates reveals role in maturation of intron-encoded box C/D small nucleolar RNAs.||PubMed Central||Coughlin, D.J., J.A. Pleiss, S.C. Walker, G.B. Whitworth, D.R. Engelke. (2008) PNAS 34: 12218-23.|
|Rapid, Transcript-Specific Changes in Splicing in Response to Environmental Stress||PubMed Central||Pleiss, J.A.*, G.B. Whitworth*, M. Bergkessel and C. Guthrie. (2007) Rapid, Transcript-Specific Changes in Splicing in Response to Environmental Stress. Molecular Cell 27: 928-937.|
|Transcript Specificity in Yeast Pre-mRNA Splicing Revealed by Mutations in Core Spliceosomal Components||PLoS Biology||Pleiss, J.A., G.B. Whitworth, M. Bergkessel and C. Guthrie. (2007) Transcript Specificity in Yeast Pre-mRNA Splicing Revealed by Mutations in Core Spliceosomal Components. PLoS Biology 5: e90|